Are All Clonal Crops Equally Vulnerable To Insect Pest Attacks? The Avocado File

In wild populations, most plant species exhibit high genetic diversity, which often is manifested as high chemical diversity and variation. This chemical heterogeneity is very important in plant populations to resist insect and pathogen attack. However, in monoculture crops, where chemical diversity in quality and quantity is low, possibilities to resist pest attack and diseases are diminished.

It is common knowledge that genetic uniformity in annual crops leads to disastrous pest and/or pathogen attack. An emblematic example of this phenomenon is the relationship between wheat and stem rust (Puccinia graminis f.sp. tritici) where resistance genes in uniform wheat varieties were overcome by the stem rust in a few years. The epidemic caused by stem rust was answered by humans developing new wheat varieties that again favored new devastating stem rust strains.

In clonal crops, such as potatoes, avocados, bananas, apples, grapes and strawberries, genetic and chemical diversity are minimized in order to maintain yield and desirable agronomic and organoleptic characteristics. The genetic uniformity in clonal crops was one of the main causes of agro-alimentary catastrophes such as the Irish potato famine in 1845-1849. If we already know that uniformity in crops is a recipe for disaster and that variability is the antidote, how is it that clonal crops still thrive in modern agriculture, even in the organic one?

Plants produce thousands of secondary compounds (a.k.a phytochemicals) that are present as mixtures in plant tissues. Variation in concentration and composition of these mixtures can affect, positive or negatively, herbivorous insect performance (survival, foraging, feeding, oviposition, growth, weight, fertility, etc.). While some mixtures or specific compounds affect negatively growth or weight gain in generalist insects, these same compounds may be beneficial for specialist insects acting as oviposition stimulants.

The Hass avocado is a clonal crop, propagated by grafting. México harbors the biggest extension of avocado cropland, 166,000 ha, planted mainly with the cultivar Hass. The avocado tree is attacked by Copturus aguacatae, a branch-boring weevil that causes breakage of fruit-bearing branches. This weevil is endemic to Mexico and is a specialist insect that feeds only on varieties of avocados. Nowadays, it is one of the worst avocado pests because it limits fruit exportation and is potentially an invasive pest. Our team demonstrated that in the 1990s, Hass avocado orchards harbored three to thirteen tree chemotypes based on leaf terpenoids and phenylpropanoids. However, with the rapid expansion of the avocado crop, we recently detected orchards with just one tree chemotype. Is that chemical variation enough to function as a resistance factor against a specialist insect? Or is the Hass avocado crop disarmed against Copturus aguacatae?

We observed that within several orchards, some avocado trees were severely infested (many branches bored) while some of their neighbor trees remained untouched (non-bored). We hypothesized that bored and non-bored avocado trees were chemically different and thus differentially infested by Copturus aguacatae. To identify which specific compounds within the hundreds of phytochemicals in the branch tissues were related with resistance against weevil infestation, we used metabolic profiling by gas chromatography coupled to mass spectrometry. This technique allows the simultaneous analysis of a great number of compounds.

We sampled more than one hundred avocado trees in seven orchards in two seasons (rainy and dry), obtaining 210 chromatographic peaks. Contrary to our expectations, one leaf chemotype predominated in the orchards, suggesting that high phytochemical homogeneity within and among orchards prevailed. We found variation in branch phytochemistry between the rainy and dry seasons that obscured the differences between bored and non-bored trees. After controlling seasonal variation, we found many compounds differing between bored and non-bored trees; at that point, it was impossible to distinguish which compounds increased or decreased in response to insect attack and which ones were constitutive.

To resolve this quagmire, we compared the chemical composition of bored and non-bored branches from the same tree. We identified a group of sixteen compounds that increased in bored trees as a response to larvae feeding. Then, aside from that set of induced compounds, we identified constitutive obtusilactone A and α-humulene in high concentration on non-bored trees. These results suggest that both compounds are involved in Hass avocado tree resistance to weevil attack, probably acting as deterrents.

In summary, we found several sources that maintain phytochemical variation between and within avocado clones, identifying what compounds are correlated to resistance and induced response to insect attacks. The next step is to test whether these compounds have the expected effect on the insect. In general terms, we showed that a clonal crop exhibits constitutive and induced phytochemical variation related to pest resistance. Moreover, even among individual branches of the same clone, different suites of phytochemicals are deployed as something similar to the “moving target to herbivores” posed by Adler and Karban (Am. Nat., 144 (1994), pp. 813-832).

Perennial crops, such as the Hass avocado tree, could be not so defenseless against insect pests in comparison to annual crops. We found additional sources of phytochemical variation in an apparently homogenous crop that probably constitutes a resistance trait against a specialist insect that faces a changing clonal host.

These findings are described in the article entitled Metabolic profiling of Persea americana cv. Hass branch volatiles reveals seasonal chemical changes associated to the avocado branch borer, Copturus aguacatae, recently published in the journal Scientia Horticulturae.

About The Author

Claudio Meléndez-González

Claudio is a Ph.D. student at the Universidad Nacional Autónoma de México, Institute of Ecosystem and Sustainability Research.

My major aim is to conduct research in the chemical ecology of plant-associated organisms from agroecosystems in three-trophic levels, for development of alternative plaguicides and sustainable agricultural practices.

In this way, I have wide experience in isolation and identification of endophytic and phytopathogenic fungi; Bioassay-guided isolation; natural products isolation by different chromatographic techniques (CC, TLC, PTLC, HPLC, Flash Chromatography); structural elucidation of natural products by spectroscopic (RMN 1D and 2D, UV-Vis, IR), spectrometric (MS), and Chiro-optical (RO, DC) techniques; Bioassay screening for antifungal, cytotoxic and phytotoxic activities.

Francisco J. Espinosa-García

Francisco Javier Espinosa García is an ecologist specialized in chemical ecology and the ecology of invasive plants and weeds. In chemical ecology, it has made significant contributions in the description and understanding of the function of the diversity of plant secondary metabolites in the defense of plants, which are potentially useful in biological control and in the management of agroecosystems to reduce the use of pesticides In the area of weeds and invasive plants, he has produced manuals, catalogs and scientific articles widely used by colleagues, government entities, and students of agronomy and biology. It has also collaborated with the preparation and revision of the Official Standards "NOM-043-FITO-1999, specifications to prevent the introduction of quarantine weeds to Mexico" and "Project to modify the Official Mexican Standard proy-mod-nom-028- phyto-1995 establishing the requirements for the importation of grains and seeds, except for sowing. " He also participated in the elaboration of the National Strategy of Invasive Species and has contributed in the evaluation of criteria for risk analysis for introduced plant species.

Speak Your Mind!

READ THIS NEXT

The Geographic Regions Of The World

The geographic regions of the world can be divided into: Africa, Asia, Central America, Eastern Europe, European Union, Middle East, North America, Oceania, South America, and the Caribbean. There are many ways to organize the world and every single country in it. One of the ways in which to do that is by geographic regions. […]

The Sunniest Spots On The Moon Are Not Where You’d Expect Them

The 23.4° tilt of the Earth’s rotation axis with respect to the ecliptic plane is the reason for our pronounced seasons on Earth. The ecliptic plane itself is defined by Earth’s orbit around the Sun. Contrary to Earth the lunar spin axis is almost perpendicular (1.5°) to that plane leaving the Moon with no distinct […]

NDH-2: An Alternative Respiratory Enzyme

Living cells rely on the transmembrane difference of electrochemical potential to store the energy needed for their biochemical reactions. Membrane potential is generated by respiratory chain proteins, which use the energy released during the oxidation of energy-rich substrates to translocate ions across the membrane against their electrochemical gradient. The transmembrane difference in electrochemical potential is […]

Data-Driven Methods To Optimize Building Energy Use

Buildings take a central place in human life. They are globally ubiquitous and are significant consumers of energy. In 2010, the building sector accounted for 19% of all global greenhouse gas (GHG) emissions and 32% of global final energy use, according to the IPCC. Research shows that for conventional buildings, the operational phase accounts for […]

Reducing The Accumulation Of Limescale In Ultrasonic Steam Irons

Various objects have been used for thousands of years to remove wrinkles. Metal pans filled with hot coals were used for smoothing fabrics in China in the 1st century BC. The early electric irons had no easy way of controlling their temperature, and the first thermostatically controlled electric iron appeared in the 1920s. Later, steam […]

Tackling Embedded Sensing Using Soft Robotics And Machine Learning

The human body maintains a sense of its own position and motions deriving information from multiple sensory modalities working together. They include the visual, vestibular, and somatosensory system. Among these, the human somatosensory system is the most challenging to emulate in a robotic system. The somatosensory system uses internally embedded receptors to estimate body configuration […]

Electronics Sustainability: Building Circuits In Cellulose Paper With 10x Better Performance

A printer can print text and images, but also electronics. This new emerging printed electronics technology, which takes advantage of the existing graphics printing industry to produce flexible, stretchable, lightweight and wearable devices, constitutes a rapidly growing area of research, with the potential of reforming the electronics industry and changing our daily life. Paper is […]