ADVERTISEMENT

Platelets Are Crucial Regulators Of Tumor Growth And Metastasis

Platelets are anucleate components of the blood, which are derived from megakaryocytes in the bone marrow. Platelets are necessary for hemostasis, a process which causes bleeding to stop, and play an important role in wound healing. Regulating hemostasis is crucial to avoid excessive blood loss or clotting. In addition to hemostasis, platelets are active players in many steps of tumorigenesis including tumor growth, extravasation of tumor cells, and metastasis.

The concept of platelet involvement in tumor growth and metastasis is old. In the 1860s, Armand Trousseau and Leopold Riess were the first ones to describe an association between thrombosis and cancer. About 100 years later, Gabriel Gasic provided the first biological evidence of decreased metastasis after reducing blood platelet counts in mice. In a retrospective study, almost 40% of ~ 140,000 patients with thrombocytosis (i.e. > 400.000 platelets per µl of blood), had an occult malignancy, mainly of the stomach, pancreas, ovary, colon, and lung — suggesting that an increased blood platelet count could serve as a biomarker or predictor of cancer.

ADVERTISEMENT

At the molecular level, thrombocytosis can result from IL-6 secretion by tumor cells that stimulate the production of thrombopoietin in the liver and enhances the production and differentiation of megakaryocytes in the bone marrow, thereby increasing platelet counts in the peripheral blood. Cancer patients with thrombocytosis tend to have a significantly worse overall and progression-free survival, and this is only partly due to an increased risk of thromboembolic complications in these patients.

Using multiple in vitro and in vivo experiments, we showed that platelets increased proliferation of cancer cells inside tumors and just a moderate reduction in platelet counts reduced tumor size by more than 50% in murine models of ovarian cancer. We also found that platelets promoted tumor growth after withdrawal of anti-angiogenic therapy with bevacizumab (humanized monoclonal antibody directed against vascular endothelial growth factor (VEGF- A).

Anti-angiogenic therapy was developed based on Judah Folkman’s discovery that tumor growth is dependent on neovascularization, the formation of new blood vessels. However, treatment with bevacizumab or other inhibitors targeting the VEGF receptors showed only modest improvement in patient survival. Interestingly, positive effects on progression-free survival coincided only with duration of anti-angiogenic treatment and progression-free survival curves collapsed soon after cessation of therapy.

We found that platelets were critical for rebound of tumor growth after withdrawal of antiangiogenic therapy and this effect could be blocked by reducing blood platelet counts. Our results, published in The Journal of Clinical Investigation, revealed that platelets actively infiltrated into the tumor microenvironment where they came in direct contact with cancer cells to increase their proliferation and reduce their apoptosis rates. Platelet infiltration was regulated by focal adhesion kinase (FAK) in platelets, suggesting that dual targeting of FAK and VEGF could have therapeutic implications for cancer management.

ADVERTISEMENT

Despite being important for primary tumor growth, we additionally demonstrated that platelets increased anoikis resistance of cancer cells. Anoikis is a form of apoptotic cell death that occurs after cells detach from their surrounding microenvironment and resistance to anoikis is a prerequisite for metastasis because it helps tumor cells to survive in the bloodstream. In a study, published in Nature Communications, we investigated signaling pathways responsible for platelet-induced anoikis resistance in cancer cells and identified the oncogene YAP1 as the most important regulator of thrombocytosis-enhanced metastasis in murine models of ovarian and colorectal cancer. Specifically, we found that platelets induced YAP dephosphorylation via the PP1-MYPT1 phosphatase and nuclear translocation leading to interaction with the TEAD transcription factor family and transcription of genes responsible for proliferation, migration, and invasion of cancer cells. Therefore, targeting YAP1 or cellular protein phosphatases in cancer could be an avenue to hamper metastasis.

Targeting platelets in cancer therapy either by reducing platelet blood counts or functions or by interfering with platelet-tumor cell interactions can offer unique opportunities for improving cancer patient outcomes. Such approaches would ideally avoid impacting the physiological roles of platelets in hemostasis. Therefore, deepening our understanding of how platelets exit tumor blood vessels, come in contact with tumor cells, and alter tumor cell behavior, as well as bioinformatics approaches that take into account both beneficial and negative platelet effects, are essential to improve platelet-targeting interventions in cancer patients.

These findings are described in the article entitled The Platelet Lifeline to Cancer: Challenges and Opportunities, recently published in the journal Cancer CellThis work was conducted by Monika Haemmerle from the University of Texas MD Anderson Cancer Center and Martin Luther University Halle-WittenbergRebecca L. Stone from Johns Hopkins Hospital, and David G. Menter, Vahid Afshar-Kharghan, and Anil K. Sood from the University of Texas MD Anderson Cancer Center.

Comments

READ THIS NEXT

Technological Advances In Flexible Energy Storage Devices And Flexible Electronics

Flexible electronics has emerged as one of the most potential technologies that could revolutionize the modern society, including military defense, […]

Do Penguins Have Knees And Feathers?

While it may not look like it, yes, penguins DO have knees that sometimes are hidden within their short legs and […]

Ancient Cheese Found Preserved In Egyptian Tomb

Hidden by the sands of time in a cracked clay jar, a dried and chalky white substance wrapped in a […]

Dodo Bird Meets Goldilocks: Psychotherapy And The Placebo Effect

In Alice in Wonderland (Chapter III, “A Caucus-Race and a Long Tale”), Alice viewed a race among all animals initiated […]

Enhancing Gypsum Precipitation In A Compacted Montmorillonite Using An Electrokinetic Method

Despite advances in solution chemistry, little is known about the chemical reactions in confined porous media like compacted clay, where […]

Is NH3 Polar Or Nonpolar?

Ammonia, chemical formula NH3, is a colorless gas frequently used in the production of fertilizer, as a cleaning chemical, and […]

Water Deposition And Possible Water Cycles On Comet 67P/Churyumov-Gerasimenko

Whether called “dirty snowballs” or “icy dirtballs,” comets are rich in water ice. However, until today, little is known about […]

Science Trends is a popular source of science news and education around the world. We cover everything from solar power cell technology to climate change to cancer research. We help hundreds of thousands of people every month learn about the world we live in and the latest scientific breakthroughs. Want to know more?