ADVERTISEMENT

Antioxidant Treatment To Block Lung Inflammation Caused By Cigarette Smoke

Smoking is a harmful addiction responsible for several lung pathologies caused by toxicity of the cigarette smoke. One of them is chronic obstructive pulmonary disease (COPD), characterized by a chronic cough, respiratory secretions, and progressive dyspnea and fibrosis, produced by chronic exposure of susceptible individuals to cigarette smoke (CS).  In addition to cigarette smoke, other factors such as high exposure to dust, chemicals, and genetic factors could promote COPD.

Although of a different etiology, COPD and Cystic Fibrosis (CF) share similar respiratory symptoms. CF is a genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a chloride channel. In both diseases, a strong inflammatory response and oxidative stress extent constitute indicators of severity of these diseases. The knowledge of the existence of a vicious circle between inflammation and oxidative stress highlight the importance of a therapeutic approach to turning off this damaging circle. Thus, the use of antioxidant drugs for the treatment of these respiratory diseases could enhance the results obtained by traditional treatments applied to patients with these disorders.

ADVERTISEMENT

In the last years, the antioxidant N-acetylcysteine (NAC) has emerged as a mucolytic, antioxidant, and anti-inflammatory drug for the treatment of respiratory diseases. To test the potential of the antioxidant treatment in COPD, we used a simple model system made with Calu-3 airway cells cultured in vitro exposed to cigarette smoke extract (CSE). The cigarette smoke contains more than 3500 compounds, many of them with toxic activity against the human organism, in many cases by inducing enhanced reactive oxygen species (ROS) production. ROS are highly oxidant molecules and when its production exceeds the antioxidant cellular defense result harmful to cell organelles, proteins, lipids, and other compounds, even reaching the DNA and producing irreversible DNA mutation that eventually produces cancer.

The exposition to CSE caused a reduction in the CFTR expression, an increase in the ROS production and in the expression/secretion of the cytokines IL-6 and IL-8, two proinflammatory markers. Interestingly, the increased secretion of these cytokines was blocked by treatment with the antioxidant NAC, suggesting that the prevention of high levels of ROS could serve as an anti-inflammatory treatment against cigarette smoke injury.

However, a fast induction of mitochondrial ROS (mtROS) and a later mitochondrial Complex I-III activity impairment was also observed, which could not be improved with NAC treatment. Thus, while the NAC effects over ROS and cytokine levels suggest that an antioxidant treatment may help to reduce inflammation in COPD, the results obtained evidence the need for an antioxidant therapy specifically directed to reduce the mitochondrial oxidative stress caused by CSE, which could not be reverted with NAC treatment. In recent years, several drugs have been reported as mitochondrial antioxidants, such as MitoQ10 or SkQ1, that could have beneficial effects on the treatment of respiratory diseases.

These findings are described in the article entitled N-acetyl cysteine reverts the proinflammatory state induced by cigarette smoke extract in lung Calu-3 cells, recently published in the journal Redox BiologyThe work was conducted by Ángel G. Valdivieso, Verónica Sotomayor, Mariángeles Clauzure, Tomás A. Santa-Coloma, in the Laboratory of Cell and Molecular Biology, Institute for Biomedical Research (BIOMED, UCA-CONICET), School of Medical Sciences, Pontifical Catholic University of Argentina (UCA) and The National Scientific and Technical Research Council of Argentina (CONICET), and by Andrea V. Dugour and Juan M. Figueroa from the Fundación Pablo Cassará, Buenos Aires, Argentina.

ADVERTISEMENT

Comments

READ THIS NEXT

Who Knew What Tau In Oligodendrocytes Can Do?

Being tuned in to the changes in life and staying current makes you young at heart. As life evolves, the […]

Correcting Computer Models Of Structures For Improved Prediction Of Dynamic Response

Engineered structures are present in almost all aspects of daily life. The houses and buildings in which we live and […]

What Is Sodium Hydrogen Carbonate?

Sodium Hydrogen Carbonate, sometimes called Sodium bicarbonate, and commonly known as baking soda is a common chemical compound with a […]

Algal Turf Scrubbing: Creating Helpful, Not Harmful, Algal “Blooms”

Thanks to the “green revolution,” food has never been cheaper or more abundant. Ironically, high-performance agriculture threatens our future ability […]

Cinder Cone Volcanoes: With Examples

A cinder cone volcano is a steep-sided conical volcano characterized by loose rock fragments from eruptions. These fragments include pyroclastic […]

What Is The Derivative Of e^2x?

The derivative of a function is a function that tells you the rate of change of the initial function at […]

Restored Oxbows As A Nutrient Reduction Practice In The Agricultural Midwest

Nutrient export from the Corn Belt of the United States is impacting rivers at local and regional scales and is […]

Science Trends is a popular source of science news and education around the world. We cover everything from solar power cell technology to climate change to cancer research. We help hundreds of thousands of people every month learn about the world we live in and the latest scientific breakthroughs. Want to know more?