DNA Combing Technology: A Simple But Useful Technique
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Hemendra is a research scientist at the University of Rajasthan.

                       

DNA Combing Technology: A Simple But Useful Technique

DNA combing is a simple technique which can be used for visualization of single DNA molecules by following simple steps. A single DNA molecule is 2 nm wide but when intercalated by a fluorescent dye, it can be seen with the help of charge coupled device camera. To perform DNA combing pure genomic DNA is extracted from tissues and is mixed in combing buffer solution carrying TE, MgCl2 ions, a fluorescent dye such as SybrGold or SybrGreen and a surfactant...

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